Microsatellites Obtained Using Strand Extension: An Enrichment Protocol
نویسندگان
چکیده
منابع مشابه
Microsatellites obtained using strand extension: an enrichment protocol.
A new method is described to enrich genomic libraries for clones containing microsatellite repeats. The method involves selection on completed M13 genomic libraries rather than on genomic DNA before library construction. It uses two reactions, in which microsatellite oligonucleotides prime strand extension. The first reaction uses a biotinylated primer allowing vectors with microsatellite-conta...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1999
ISSN: 0736-6205,1940-9818
DOI: 10.2144/99264st05